Journal: Applied Microbiology and Biotechnology

Article Title: Characterization and evaluation of a novel polylactic acid-made bioreactor for large-scale adherent cell expansion

doi: 10.1007/s00253-026-13880-4

Figure Lengend Snippet: Attachment kinetics of CT-26 WT cells to CellScrew® mini surface. CT-26 WT cells were seeded into CellScrew® mini at a cell density of 1 × 10E4 cells/cm 2 and attachment was monitored by taking supernatant at indicated time points. ( A ) shows attached cells over time at 2 rpm rotational speed. ( B ) shows glucose (black square) and lactate concentration (black triangle) of supernatant over time. Values represent the mean ± SD of n = 3 biological replicates; in some cases, error bars are smaller than the symbols and therefore not visible

Article Snippet: The murine melanoma cell line B16-F10 (CRL-6475, RRID: CVCL_0159, C57BL/6 background, ATCC, Manassas, VA, USA) and the murine colon carcinoma cell line CT-26 Wild-Type (CT-26 WT; CRL-2638, RRID: CVCL_7256, BALB/c background, ATCC, Manassas, VA, USA) were used in this study.

Techniques: Concentration Assay

Journal: Applied Microbiology and Biotechnology

Article Title: Characterization and evaluation of a novel polylactic acid-made bioreactor for large-scale adherent cell expansion

doi: 10.1007/s00253-026-13880-4

Figure Lengend Snippet: Growth kinetics of CT-26 WT cells. CT-26 WT cells were cultivated in CellScrew® mini at 2 rpm rotational speed for 96 h. The seeding concentration for CT-26 WT cells was 1 × 10E4 cells/cm 2 . ( A ) shows the cell density [x 10E4] harvested per cm 2 over time. ( B ) shows glucose (black square) and lactate concentration (black triangle) in supernatant over time. Data are presented as mean (± SD) of n = 3 per time point; in some cases, error bars are smaller than the symbols and therefore not visible

Article Snippet: The murine melanoma cell line B16-F10 (CRL-6475, RRID: CVCL_0159, C57BL/6 background, ATCC, Manassas, VA, USA) and the murine colon carcinoma cell line CT-26 Wild-Type (CT-26 WT; CRL-2638, RRID: CVCL_7256, BALB/c background, ATCC, Manassas, VA, USA) were used in this study.

Techniques: Concentration Assay

Journal: Applied Microbiology and Biotechnology

Article Title: Characterization and evaluation of a novel polylactic acid-made bioreactor for large-scale adherent cell expansion

doi: 10.1007/s00253-026-13880-4

Figure Lengend Snippet: Reduction and replacement of dissociation reagent on PLA surface. Harvest efficiency [%] and cell viability [%] of B16-F10 ( A ) and CT-26 WT ( B ) cell lines after 96 h of culture on PLA surface in the CellScrew® mini. Cells were detached using 100% Accutase, 50% Accutase + 50% DPBS, or 100% Envzyme™ Super under identical conditions ( n = 1)

Article Snippet: The murine melanoma cell line B16-F10 (CRL-6475, RRID: CVCL_0159, C57BL/6 background, ATCC, Manassas, VA, USA) and the murine colon carcinoma cell line CT-26 Wild-Type (CT-26 WT; CRL-2638, RRID: CVCL_7256, BALB/c background, ATCC, Manassas, VA, USA) were used in this study.

Techniques:

Journal: Communications Biology

Article Title: The MYB-related transcription factor MYPOP acts as a selective regulator of cancer cell growth

doi: 10.1038/s42003-026-10272-2

Figure Lengend Snippet: a Protein expression of untreated, control mRNA and MYPOP mRNA-transfected CT-26 cells and B16-F10 cells at the indicated time points was analyzed by western blotting using anti-MYPOP and anti-GAPDH antibodies. b Cell counts of untreated, control mRNA and MYPOP mRNA-transfected CT-26 cells and B16-F10 cells at the indicated time points. Mean values ( n = 3) are shown as a line diagram + individual data points. Statistical significance was determined comparing Control and MYPOP cell counts at 54 h p.t. with p = 0.0650 for CT- 26 and with p = 0.0313 for B16-F10.

Article Snippet: The murine CT-26 cell line (ATCC, CRL-2638) was cultured in RPMI Medium 1640 (Thermo Fisher Scientific, USA) supplemented with 10% FBS (SUPERIOR stabil, Bio&Sell).

Techniques: Expressing, Control, Transfection, Western Blot